PROTEOMIC APPROXIMATION OF DIFFERENTIAL EXPRESSION IN Pleurotus ostreatus (OSTRA FUNGUS), AS A RESPONSE TO BIO-ACUMULATION OF LEAD (PB). (#904)

Authors

HUARAYA-CHAMBI, F.R

CALCINA-RONDAN, L.E

Abstract

In recent years there has been considerable progress in environmental biotechnology, in the use and application of different living organisms for the bioremediation of different compounds, many times of toxic nature in contaminated sites or media, these bioremediation processes offer high specificity in the removal in both in situ and ex-situ systems. The use of white rot fungi in heavy metal bioremediation processes is a very promising environmental technology, whose studies focus on the ability to degrade persistent compounds. The purpose of the research is to try to decipher which are the key elements of the molecular genetics of Pleorotus ostreatus that make it tolerant and allow its accumulation of heavy metals such as lead (Pb), from a differential proteomic approach perspective. The methodology is divided into three stages: 1.- purification; obtaining the crude extract, precipitation with 90% ammonium sulfate, and hydrophobic interaction chromatography. Tandem mass spectrometry ESI-CID-MS/MS 3.- Bioinformatic analysis using DNA Star software, with which sequential homology studies were performed to know the structural character of the proteins. Through the results of the proteomic approach, we have established that manganese-peroxidase oxidative enzymes are found in their expression levels, as evidenced in the studies of the proteins. Through the results of the proteomic approach, we have been able to establish that manganese peroxidase oxidative enzymes are found in their expression levels, as evidenced in the sequence homology studies, showing a high degree of homology and identity of conserved regions, in the studied enzymes of Pleurotus ostreatus, in whose alignments of the sequenced peptides with high sequence homology, we can suggest that these enzymes are essential in the bioremediation process