Latin American and Caribbean Consortium of Engineering Institutions
Conference Track: Emerging
Technologies
Keywords:
fluorescence microscopy, multiphoton microscopy, spectrometer,
femtosecond pulsed laser
Contact Title: Prof.
Contact First Name: Ching-Hua (Dennis)
Contact Last Name: Fan
University:
Florida International University
Web:
http://faculty.eng.fiu.edu/~dfan/
Position:
Assistant Professor
Country:
USA
Email:
dfan@fiu.edu
Fax:
1-305-348-1932
Paper Title:
Ching-Hua.
Fan†,
Ph.D.
Department
of Mechanical and Materials Engineering
Florida International University
Miami, FL 33174, USA
Abstract
In this work, a modern biomedical instrument—multiphoton laser scanning microscope (MPLSM)—is used to image biological specimens by inducing fluorescence from stained fluorophores or tagged fluorescence proteins both in vitro and in vivo. By integrating with a time-correlated single-photon counting (TCSPC) detector and a lifetime measurement module, the modified MPLSM system extracts further optical information—fluorescence lifetime—from the fluorescent emission of the specimens. Lifetime images of several biological specimens are presented, showing that the lifetime information extracted from the fluorescence signals can serve as indicators to identify the biological, physical, and chemical microenvironments of the fluorophores, e.g., biochemical constituents, pH value, and temperature. Moreover, lifetime images at various emission wavelengths are collected using a concave optical grating, providing additional optical information from the same biological specimen. The presented biological imaging technique exhibits promising potentials for human diseases diagnosis.
The authors gratefully
acknowledge the support for this work by Prof. J. G. White and Mr. K. W. Eliceiri
at UW-Madison. Also, the
authors appreciate Dr. Al Kutchera for specimen preparation and the
valuable comments from Dr. Jayne Squirrell, Dr. Fern Finger, and Prof.
Nirmala Ramanujam.
†
Assistant Professor. Author
to whom correspondence should be addressed.
E-mail: dfan@fiu.edu. Tel: (305) 348–4027, Fax: (305) 348–1932