Latin American and Caribbean Consortium of Engineering Institutions

Conference Track:  Emerging Technologies

Selection1:       Full Session

Language:         English

Keywords:         fluorescence microscopy, multiphoton microscopy, spectrometer, femtosecond pulsed laser

Contact Title:    Prof.

Contact First Name: Ching-Hua (Dennis)

Contact Last Name:  Fan

University:       Florida International University

Web:              http://faculty.eng.fiu.edu/~dfan/

Position:         Assistant Professor

Country:          USA

Email:            dfan@fiu.edu

Fax:              1-305-348-1932

Paper Title:

Wavelength-resolved fluorescence lifetime imaging using multiphoton laser scanning microscopy

Abstract:

This work presents a novel combined spectral and lifetime multiphoton microscopy technique to further image and analyze biological specimens in vitro.   Florescence emission from biological specimens is induced by a Coherent DMP-1000 Nd:YLF mode-locked laser oscillator with a nominal wavelength of 1047 nm, a pulse duration of ~100 fs, a maximum pulse energy of 7 nJ, and a repetition rate of 120 MHz.  The induced fluorescence emission is collected by a high numerical aperture objective lens (NA = 1.4) and sent to a 16-wavelength-channel lifetime PMT through a concave optical grating.  The different wavelength components separated by the grating are directed to different channels of the lifetime PMT to form high resolution wavelength-resolved lifetime images. This new optical technique integrates several cutting-edge technologies, including laser scanning microscopy, nonlinear multiphoton imaging, spectral analysis, and lifetime imaging, to resolve additional significant biolo!

gical information from the laser-induced fluorescence emission.  The presented microscopy imaging technique also exhibits very promising potentials in other research areas, such as materials science, microscale heat transfer, and human disease diagnosis.

Mailing Address:

10555 W. Flagler St., EAS 3466

Miami,FL 33174

Phone:

1-305-348-4027

Other Authors:

Ching-Hua (Dennis) Fan / dfan@fiu.edu / Florida International University /(P)